Regemat REG4Life BioPrinter

Key Features:

• Modular multi-material printing (hydrogels, thermoplastics, pastes)
• Automated calibration with laser precision
• Independent Z-axis movement for printing on various substrates
• Advanced temperature control: Heated syringes (0-50°C), extendable to 250°C with metallic or glass syringes

Modular and Customisable System

Each research project requires a specific configuration because no universal bioprinting system suits all applications. At Regemat 3D, we develop a customised Reg4Life version for each research group and project. The Reg4Life system uses a modular design that you can adapt for future studies without buying new equipment. Its Plug & Play print-head system lets you exchange heads quickly and easily for each printing process.

Equipment Design

We built the Reg4Life entirely in PVC to reduce the weight to under 30 kg. Lightweight and grip handles allow easy transport and placement inside a laminar-flow cabinet. This placement improves aseptic working conditions beyond what HEPA filters provide. Our engineering team designed the bioprinter to promote upward laminar flow throughout the system.

A closed side compartment isolates filament rolls so they never disrupt laminar flow. We use rounded shapes to avoid corners where dirt and bacteria might accumulate. These features make cleaning and disinfection faster and easier. PVC covers support chemical disinfection, thermal shock treatments and UV or gamma sterilisation.

We constantly refine the system to ensure GMP-compliant, aseptic operation suitable for clinical translation.

Print Head

The new Reg4Life print head provides precise independent movement control across the X, Y and Z axes. Three Plug & Play print heads allow fast exchange of syringes and printing tools. A double thermoplastic extruder comes standard and prints two filaments during one process.

LED-Light System

The integrated LED system guides users through each experimental stage with a three-colour code.

• Blue indicates readiness to bioprint.
• Green indicates active bioprinting.
• Red indicates a paused process.

Automatic Calibration System

A laser sensor system performs automatic, precise calibration of all tools without user intervention. Software adjustments enable even finer calibration for different syringe types and nozzle diameters.

Syringes and Tools

Our syringe system reflects over five years of global collaboration with research teams. This experience allows broad compatibility with diverse materials and bioinks. Reg4Life is the only system offering a refrigeration module operating from –20°C to 100°C.

• Two-component syringes extrude mixed materials to form homogeneous composites.
• Refrigerated syringes maintain stable material temperatures across the entire surface.
• Metallic syringes provide uniform heating up to 250°C with electronic filament control.
• High-temperature metal syringes ensure homogeneous heat distribution during extrusion.
• Heated syringes maintain temperatures up to 50°C.

Print Bed and Bioprinting Surfaces

• Automatic X, Y and Z calibration ensures precise control of material printing height.
• Independent Z-axis movement enables printing on various surfaces.
• A standard glass bed provides a 220 × 160 × 110 mm working area.
• This area supports constructs measuring up to 150 × 160 × 110 mm.
• Adapters allow printing on Petri dishes and 6, 12, 24, 96 and 128-well plates.
• The heating bed controls temperatures from room temperature to 120°C.
• Optional cooling extends the range from –20°C to 120°C.

Technology

We optimised the Reg4Life system to improve the entire bioprinting process. The hardware and software support precise control of FDM and IPF technologies. Reg4Life is the only commercial system validated for Regemat’s IVF technology.

FDM

FDM models scaffolds with complex external geometry and meshed internal structures. The extruder melts thermoplastic material and deposits it layer by layer.

IPF

IPF selects specific layers and injects cells directly into chosen pores. The system injects controlled cell volumes that may differ between layers. IPF supports cell survival under high-temperature thermoplastic conditions.

IVF

Regemat 3D exclusively developed IVF for complete bioink injection across scaffold volumes. IVF ensures full layer filling and supports small-volume work, including osteochondral repairs.

Software

Regemat 3D developed the Reg4Life software to control every bioprinting parameter precisely. Users can customise configurations for each team and each bioprinting process. Regemat 3D Designer prints multiple objects simultaneously, each with independent settings.

Object Pre-Visualization

Our intuitive software lets users design structures or import STL geometries. A preview tool enables configuration of the internal mesh and numerous printing parameters.

Features

• Users can load and view STL models interactively.
• They can configure printing phases and parameters using G-code.
• The software supports extensive scaffold parameter customisation.
• Users can generate and inspect layered print paths interactively.
• Injection settings include point, layer and injection volume.
• The system prints on multiple platform types.

Reg4Life Components

Reg4Life includes laser-based automatic calibration, a heated syringe, a heated bed and a double extruder. We offer additional configurations to meet the specific needs of each research team. We also design custom tools and software features when required. Our dual role as engineers and researchers allows full system customisation for collaborators.

Tool Descriptions

• Bi-component syringes extrude two mixed materials to create composite structures.
• Refrigerated syringes maintain stable temperatures from –20°C to 100°C.
• Metallic syringes heat uniformly up to 250°C with electronic filament control.
• High-temperature extruders ensure even heat distribution across the extrusion pathway.
• UV and blue-light curing tools deliver 14,000 mW/cm² between 365 and 405 nm.
• A double thermoplastic extruder prints two filaments during the same process.
• Heated and cooled beds maintain uniform temperatures across the entire surface.
• Laser sensors provide high-precision automatic nozzle positioning.